The 1,700 square foot (157 square meter) suite will be available as of next month for $9,500 per night.
Guests staying at the Bentley Suite will have complimentary access to a 2013 Bentley Mulsanne ride within a 10-block radius of the Fifth Avenue hotel.
The hotel already offers complimentary Bentley chauffeur service to suite guests in its properties all around the world.
It is not the first time the St. Regis Hotel, owned by Starwood, has collaborated with a luxury brand. The same property in New York has offered guests a Tiffany suite since June 2011 and a Dior suite since September 2011.
The St. Regis Hotel in Florence unveiled a Bottega Veneta suite designed by Bottega Veneta’s creative director, Tomas Maier, using furniture from the Bottega Veneta home collection in 2007.
Other collaborations between high-end auto brands and luxury hotels include the Jaguar suite at 51 Buckingham Gate, Taj Suites and Residences in London unveiled last year.
The design for the 1,832 square foot bedroom features classic and contemporary Jaguar models including the legendary 1960s E-type and the C-X75.